Part:BBa_K3526004

tac pro-atzR-T4 ter

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 1026
  Illegal AgeI site found at 864
  
• 1000
  COMPATIBLE WITH RFC[1000]

A transcription factor, atzR, which can sense the presence of cyanuric acid.

Contribution

BBa_K3526004 is a composite part that contains a special transcription factor AtzR. This part should be used with the part BBa_K3526005. Under the presence of cyanuric acid (CYA), the AtzR can bind CYA and regulate the promoter PatzD. As a result, the GFP is expressed and emit green fluorescence.

Figure 1

Figure 2

Engineering Success

To test the function of part BBa_K3526004, we co-transform the part BBa_K3526004 and part BBa_K3526005 into Pseudomonas putida KT2440. After reading related articles, we choose 0μM, 50μM, and 100μM as the detection concentrations of CYA. After preliminary analysis, we obtained Figure 3.

Figure 3

The results of the experiment show that with the presence of part BBa_K3526004 and BBa_K3526005, Pseudomonas putida KT2440 can detect cyanuric acid.